Questions on Move Part

Questions on Move Part

If Nate can answer this, I would greatly appreciate it.

I have a round flat part that I have to check. Datum B is a diameter; I can access it only on one side. Several holes on the other side (bottom) are called back to Datum B. I realize I can use Move Part. My question is after I flip the part over, can I recall Datum B even if it's on the other side? I can't measure it again because it's not accessible. 
Hope this makes sense.

Thanks for your help